The delta strain of the new coronavirus is raging around the world. Another new coronavirus mutant, lambda, has struck again. Faced with the constantly mutating virus, scientists are still working around the clock on the pathogenesis of the virus, potential therapeutic targets and screening for effective antibodies.


single cell research


Cell research is one of the hotspots in the current biotechnology field. The object of traditional cell biology analysis is usually hundreds of thousands to millions of cells. With the development of modern biology, scientists tend to study the diversity that exists between cells

This can not only fully understand some special cell functions in cell populations, but also identify a small amount of diversity in a large number of cell populations, providing a new technical approach for early diagnosis and treatment of major diseases, drug screening and cell-cell interaction research.


Microfluidic single-cell analysis technology


In recent years, laboratories have often used microfluidics for single-cell research, and in microfluidic single-cell analysis, single-cell capture is also required, that is, single cells are isolated in micron-scale channels and fixed in a specific position for subsequent operations and operations. Riddle

Droplet microfluidic technology provides further powerful support for the detection, sorting and analysis of single cells, that is, a series of nanoliter-to-picoliter droplets are generated through the mechanical action of two-phase or multi-phase immiscible liquids in micron-scale pipelines. .

In the single-cell encapsulation experiment using droplet microfluidics, the cell-containing buffer was used to interact with the oil phase liquid to form a uniform droplet of cell buffer in the oil phase, and each cell was encapsulated in the droplet. This technology can not only generate a large number of single-cell droplets in a short time, but also each droplet can be used as an independent single-cell microreactor, which can effectively avoid cross-contamination and meet the final needs of a variety of single-cell research.

The common droplet generation system in the experiment mainly includes three parts: micro syringe pump, microfluidic chip and high-throughput screening system. The generation of microdroplets with uniform size and stable velocity is the key to the normal operation of the system. As the power source of the whole system, the micro-injection pump provides a stable micro-liquid flow and a stable and fine power for the production of micro-droplets.
 

Microsyringe pumps, microfluidic chips and high-throughput screening systems

Applications


A medical laboratory in a domestic university conducts single-cell encapsulation experiments through droplet microfluidic technology. The laboratory needs to transmit a variety of liquids, so multiple Rongbai syringe pumps are used for accurate and stable liquid transmission.
 

Microdroplet generation process under the microscope


 

Micro droplets under the microscope

Microdroplets under the microscope

Microdroplets seen under a microscope contain cells

Rongbai new LSP02-1C laboratory syringe pump supports precise distribution. Driven by the syringe pump, the pulsation of liquid flow is small and the stability is good. It can provide stable microfluidic flow in the microfluidic system. Moreover, LSP02-1C can control multiple pumps at the same time through PC software, and each pump has independent operating parameters to meet the application of droplet microfluidic control that requires multiple pumps to cooperate with each other, helping to complete the experiment efficiently.