As a new generation of precision therapy tools, cell therapy has emerged as a hot field as researchers advance in their understanding of cellular and molecular biology, combined with technological improvements, better models of cellular diseases can be established from primary cells.

Primary cells refer to human or animal tissues or organs taken out of the body and then treated with various enzyme chelators or mechanical methods, dispersed into single cells, and then cultured in vitro. The biological characteristics of cells in primary culture have not changed much, and can reflect the growth characteristics in vivo, so it is suitable for studying the growth, metabolism and reproduction of cells in organisms, so as to develop new effective treatment methods for intervention and achieve the purpose of precision medicine.

The primary source and separation are the key

Primary cell culture mainly includes three parts: collection→isolation→culture. However, the difficulty of culture is directly related to the tissue type, the degree of differentiation, the method of primary material collection, and separation, which is also the key to determining the success or failure of primary culture. When collecting materials, attention should be paid to aseptic operation, the material collection process should be as fast as possible, and the tissue should be kept fresh.

Perfusion is one of the experimental methods for pharmacological or physiological research in medical schools and scientific research institutions. It is also a very important link in the extraction of most tissue cells in animals, which directly determines the quality of cells obtained in experiments. During perfusion, the Rongbo peristaltic pump pumps perfusate to isolated tissues or organs, so that the relevant experiments can be carried out smoothly. At the same time, the pump can be adjusted and controlled by setting the perfusion mode, perfusion speed, perfusion volume, etc. to suit different research needs.

In addition, due to the tight association of various cells in humans or animals, it is not conducive to the growth and reproduction of individual cells in vitro, so the existing tissue pieces must be fully dispersed to dissociate the cells.

Case Studies

A laboratory uses the Rongbai BT100FY peristaltic pump to extract primary rat liver cells. During the extraction process, the perfusion fluid is perfused from the portal vein of the rat liver at a flow rate of 20ml/min to flush out the blood including blood cells. .

The rat liver was then dissociated into single cells by collagenase at a flow rate of 10 ml/min. In the experiment, it is necessary to perfuse at different flow rates in two steps. The BT100FY peristaltic pump is equipped with a speed control knob for easy flow rate setting.

Users said: Rongbai's peristaltic pump can provide accurate and repeatable flow, and the pump has experienced a variety of complex environments, the purchase time is long, the manual has turned yellow, but it is still competent for perfusion work, which is worthy of praise.

Rongbai distribution type peristaltic pump BT100FY has two working modes: flow and distribution. A wide flow range of 0-380mL/min is available in flow mode. In the dispensing mode, it can automatically perform multiple repeated filling and dispensing operations, and the filling accuracy can reach 1%.

Moreover, the Rongbai peristaltic pump has reliable quality and stable performance, which can ensure the rapid replacement of the perfusate in the perfusion tank, maintain the good physiological state of the isolated tissues and organs, and enable the experiment to proceed smoothly. When transferring fluids, the fluids only come into contact with the inner wall of the hose. By using hoses that meet hygienic requirements, the sterility requirements of perfusion experiments are met.

In addition, the Rongbai peristaltic pump has good self-sealing performance, and the pipeline can be effectively sealed without a valve on the pipeline to prevent the backflow of the perfusate. Rongbai constant flow pump has been committed to providing better solutions for scientific researchers in the fields of life science, biomedicine, R&D, analysis and testing.