Fermentation is a biochemical reaction that people are exposed to earlier. The process of producing and accumulating a large number of metabolites that people need by carrying out large-scale growth and cultivation of biologically active substances (microbial animal and plant cell enzymes, etc.) is called "fermentation". ". Today it is widely used in the food industry, biological and chemical industries. As a deep cultivator in the field of precision fluid transmission, Rongbai constant flow pump has also made a lot of contributions to the field of life science, especially solving many challenges in fermentation applications!

The fermenter (or various reactors) is the core equipment of fermentation, which provides a good reaction environment for the fermentation process. The structure of the fermenter mainly includes two parts: the reaction vessel and the control system. The reaction vessel provides the reaction environment of the fermentation process. The control system will react Environmental controls are at set target values.

It is generally believed that: 500L or less is a laboratory-scale fermenter, and more than 500L is a production-scale fermenter.

Laboratory small fermenter fermentation is the prototype and basis of industrial fermenter fermentation, providing data and information for the operation of production-scale fermenters, including: seed validation, medium formula screening, optimization of process parameters, etc. Fermentation is the only way to put into industrial production in the future!

Laboratory-scale fermenters are generally equipped with four-way peristaltic pumps for each reaction vessel. Generally, OEM products of fixed-speed peristaltic pumps are used. The control system controls the amount of addition by controlling the start and stop times of the peristaltic pumps. The typical product is Rongbai's OEM03-TH15 product

Among them, two peristaltic pumps add acid and alkali: the control system monitors the pH value to add acid or alkali to the closed-loop control to control the pH value of the reaction environment at the set target value

One-way peristaltic pump to add defoamer: the control system monitors the foam through closed-loop control of adding defoamer to control the amount of foam in the reaction environment at the set target value

The other peristaltic pump adds medium: the control system monitors the nutrient level through the closed-loop control of adding nutrient reagents to control the nutrient level of the reaction environment at the set target value

In the fermenter fermentation test, the fermentation medium is used for the growth and reproduction of strains and the synthesis of products. It not only enables the seeds to grow rapidly after inoculation to reach a certain mycelial concentration, but also enables the grown cells to rapidly synthesize the desired products.

Therefore, it is required that the composition of the fermentation medium should be rich in complete carbon and nitrogen sources, and attention should be paid to the combination of quick-acting and delayed-acting nutrients. Growth factors required for bacterial growth and element precursors and promoters required for product synthesis. In addition to the elements and compounds necessary for the growth of bacteria, there are also specific element precursors and promoters required for the product.

However, if the concentration of the total carbon source, nitrogen source, phosphorus source, etc. required for growth and biosynthesis products is too high, or the optimal conditions for the two stages of growth and synthesis are different, it can be considered that the medium should be fed in batches. Satisfy.

Reagents such as antifoaming agents are also essential, because microorganisms must produce metabolites during fermentation experiments. These metabolites will cause pH changes in the bacterial liquid, and as the fermentation proceeds, a large number of bubbles will definitely be generated, which will make the bacterial liquid The decrease of dissolved oxygen leads to the failure of normal growth and reproduction of bacteria. At this time, acid and alkali are needed to adjust its pH. Defoamer is used to eliminate a large number of air bubbles to achieve the most suitable growth environment.

solution

Since lab-scale fermenters will perform a variety of experiments, it is often necessary to change the type of added liquid, while the liquid delivered by the Rongbo peristaltic pump only touches the hose. This kind of application can be met with a simple change of the hose. A better choice.

Small fermenters for laboratories can usually use Rongbai OEM03-TH15 peristaltic pumps, which are small in size and compact in structure. The pilot-scale fermenter requires a large flow rate, which can be manually adjusted or remotely closed-loop controlled by using a standard benchtop peristaltic pump from Rongbai.

Production-scale fermenters generally do not change the type of liquid added because the process parameters are fixed. There are other liquid addition methods, but peristaltic pumps are still one of the important methods, especially for shear-sensitive liquids. Choose a peristaltic pump with high flow.

Rongbai constant flow pump has a complete peristaltic pump product line, including: desktop peristaltic pump standard products, fixed speed peristaltic pump OEM products, user-controlled peristaltic pump OEM products, user-driven peristaltic pump OEM products, to meet the needs of customers in various scenarios.